Selected Publication
1: Verdegaal EM, de Miranda NF, Visser M, Harryvan T, van Buuren MM, Andersen RS,
Hadrup SR, van der Minne CE, Schotte R, Spits H, Haanen JB, Kapiteijn EH,
Schumacher TN, van der Burg SH. Neoantigen landscape dynamics during human
melanoma-T cell interactions. Nature. 2016 Aug 4;536(7614):91-5. PubMed PMID:
27350335.
2: Glenthøj A, Ørskov AD, Hansen JW, Hadrup SR, O’Connell C, Grønbæk K. Immune
Mechanisms in Myelodysplastic Syndrome. Int J Mol Sci. 2016 Jun 15;17(6). pii:
E944. doi: 10.3390/ijms17060944. Review. PubMed PMID: 27314337; PubMed Central
PMCID: PMC4926477.
3: McGranahan N, Furness AJ, Rosenthal R, Ramskov S, Lyngaa R, Saini SK,
Jamal-Hanjani M, Wilson GA, Birkbak NJ, Hiley CT, Watkins TB, Shafi S, Murugaesu
N, Mitter R, Akarca AU, Linares J, Marafioti T, Henry JY, Van Allen EM, Miao D,
Schilling B, Schadendorf D, Garraway LA, Makarov V, Rizvi NA, Snyder A, Hellmann
MD, Merghoub T, Wolchok JD, Shukla SA, Wu CJ, Peggs KS, Chan TA, Hadrup SR,
Quezada SA, Swanton C. Clonal neoantigens elicit T cell immunoreactivity and
sensitivity to immune checkpoint blockade. Science. 2016 Mar 25;351(6280):1463-9.
doi: 10.1126/science.aaf1490. Epub 2016 Mar 3. PubMed PMID: 26940869; PubMed
Central PMCID: PMC4984254.
4: Overgaard NH, Frøsig TM, Welner S, Rasmussen M, Ilsøe M, Sørensen MR, Andersen
MH, Buus S, Jungersen G. Establishing the pig as a large animal model for vaccine
development against human cancer. Front Genet. 2015 Sep 15;6:286. doi:
10.3389/fgene.2015.00286. eCollection 2015. PubMed PMID: 26442104; PubMed Central
PMCID: PMC4584933.
5: Frøsig TM. Elucidating the immunological effects of 5-azacytidine treatment in
patients with myelodysplastic syndrome and identifying new conditional ligands
and T-cell epitopes of relevance in melanoma. Dan Med J. 2015 Aug;62(8):B5144.
PubMed PMID: 26239596.
6: Frøsig TM, Yap J, Seremet T, Lyngaa R, Svane IM, Thor Straten P, Heemskerk MH,
Grotenbreg GM, Hadrup SR. Design and validation of conditional ligands for
HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05. Cytometry A. 2015
Oct;87(10):967-75. doi: 10.1002/cyto.a.22689. Epub 2015 Jun 1. PubMed PMID:
26033882.
7: Hjortsø MD, Larsen SK, Kongsted P, Met Ö, Frøsig TM, Andersen GH, Ahmad SM,
Svane IM, Becker JC, Straten PT, Andersen MH. Tryptophan 2,3-dioxygenase
(TDO)-reactive T cells differ in their functional characteristics in health and
cancer. Oncoimmunology. 2015 Jan 30;4(1):e968480. eCollection 2015 Jan. PubMed
PMID: 25949861; PubMed Central PMCID: PMC4368150.
8: Kvistborg P, Gouttefangeas C, Aghaeepour N, Cazaly A, Chattopadhyay PK, Chan
C, Eckl J, Finak G, Hadrup SR, Maecker HT, Maurer D, Mosmann T, Qiu P,
Scheuermann RH, Welters MJ, Ferrari G, Brinkman RR, Britten CM. Thinking outside
the gate: single-cell assesment in multiple dimensions. Immunity. 2015 Apr
21;42(4):591-2. doi: 10.1016/j.immuni.2015.04.006. PubMed PMID: 25902473; PubMed
Central PMCID: PMC4824634.
9: Frøsig TM, Lyngaa R, Met Ö, Larsen SK, Donia M, Svane IM, Thor Straten P,
Hadrup SR. Broadening the repertoire of melanoma-associated T-cell epitopes.
Cancer Immunol Immunother. 2015 May;64(5):609-20. doi: 10.1007/s00262-015-1664-x.
Epub 2015 Feb 18. PubMed PMID: 25854582; PubMed Central PMCID: PMC4412285.
10: Frøsig TM, Hadrup SR. Comment on “5-azacytidine promotes an inhibitory T-cell
phenotype and impairs immune mediated antileukemic activity”. Mediators Inflamm.
2015;2015:871641. doi: 10.1155/2015/871641. Epub 2015 Mar 8. PubMed PMID:
25838640; PubMed Central PMCID: PMC4370204.
11: Hadrup SR, Maurer D, Laske K, Frøsig TM, Andersen SR, Britten CM, van der
Burg SH, Walter S, Gouttefangeas C. Cryopreservation of MHC multimers:
Recommendations for quality assurance in detection of antigen specific T cells.
Cytometry A. 2015 Jan;87(1):37-48. doi: 10.1002/cyto.a.22575. Epub 2014 Oct 8.
PubMed PMID: 25297339; PubMed Central PMCID: PMC4309491.
12: Donia M, Lyngaa R, Hadrup SR. “Immunotherapy of cancer: present status and
future promise”: Danish Cancer Society Symposium, Copenhagen, Denmark, 23rd-25th
September 2013. Cancer Immunol Immunother. 2014 Sep;63(9):985-9. doi:
10.1007/s00262-014-1585-0. Epub 2014 Jul 24. PubMed PMID: 25056819; PubMed
Central PMCID: PMC4141144.
13: Gang AO, Frøsig TM, Brimnes MK, Lyngaa R, Treppendahl MB, Grønbæk K, Dufva
IH, Straten PT, Hadrup SR. 5-Azacytidine treatment sensitizes tumor cells to
T-cell mediated cytotoxicity and modulates NK cells in patients with myeloid
malignancies. Blood Cancer J. 2014 Mar 28;4:e197. doi: 10.1038/bcj.2014.14.
PubMed PMID: 24681961; PubMed Central PMCID: PMC3972700.
14: Lyngaa R, Pedersen NW, Schrama D, Thrue CA, Ibrani D, Met O, Thor Straten P,
Nghiem P, Becker JC, Hadrup SR. T-cell responses to oncogenic merkel cell
polyomavirus proteins distinguish patients with merkel cell carcinoma from
healthy donors. Clin Cancer Res. 2014 Apr 1;20(7):1768-78. doi:
10.1158/1078-0432.CCR-13-2697. Epub 2014 Feb 13. PubMed PMID: 24526738; PubMed
Central PMCID: PMC3994405.
15: Linnemann C, Heemskerk B, Kvistborg P, Kluin RJ, Bolotin DA, Chen X, Bresser
K, Nieuwland M, Schotte R, Michels S, Gomez-Eerland R, Jahn L, Hombrink P,
Legrand N, Shu CJ, Mamedov IZ, Velds A, Blank CU, Haanen JB, Turchaninova MA,
Kerkhoven RM, Spits H, Hadrup SR, Heemskerk MH, Blankenstein T, Chudakov DM,
Bendle GM, Schumacher TN. High-throughput identification of antigen-specific TCRs
by TCR gene capture. Nat Med. 2013 Nov;19(11):1534-41. doi: 10.1038/nm.3359. Epub
2013 Oct 13. PubMed PMID: 24121928.
16: Andersen RS, Andersen SR, Hjortsø MD, Lyngaa R, Idorn M, Køllgård TM, Met O,
Thor Straten P, Hadrup SR. High frequency of T cells specific for cryptic
epitopes in melanoma patients. Oncoimmunology. 2013 Jul 1;2(7):e25374. PubMed
PMID: 24073381; PubMed Central PMCID: PMC3782131.
17: Larsen SK, Munir S, Woetmann A, Frøsig TM, Odum N, Svane IM, Becker JC,
Andersen MH. Functional characterization of Foxp3-specific spontaneous immune
responses. Leukemia. 2013 Dec;27(12):2332-40. doi: 10.1038/leu.2013.196. Epub
2013 Jul 1. PubMed PMID: 23812418.
18: Lin L, Chan C, Hadrup SR, Froesig TM, Wang Q, West M. Hierarchical Bayesian
mixture modelling for antigen-specific T-cell subtyping in combinatorially
encoded flow cytometry studies. Stat Appl Genet Mol Biol. 2013 Jun;12(3):309-31.
doi: 10.1515/sagmb-2012-0001. PubMed PMID: 23629459; PubMed Central PMCID:
PMC4155753.
19: Gilchuk P, Spencer CT, Conant SB, Hill T, Gray JJ, Niu X, Zheng M, Erickson
JJ, Boyd KL, McAfee KJ, Oseroff C, Hadrup SR, Bennink JR, Hildebrand W, Edwards
KM, Crowe JE Jr, Williams JV, Buus S, Sette A, Schumacher TN, Link AJ, Joyce S.
Discovering naturally processed antigenic determinants that confer protective T
cell immunity. J Clin Invest. 2013 May;123(5):1976-87. doi: 10.1172/JCI67388.
Epub 2013 Apr 1. PubMed PMID: 23543059; PubMed Central PMCID: PMC3635741.
20: Iversen TZ, Brimnes MK, Nikolajsen K, Andersen RS, Hadrup SR, Andersen MH,
Bastholt L, Svane IM. Depletion of T lymphocytes is correlated with response to
temozolomide in melanoma patients. Oncoimmunology. 2013 Feb 1;2(2):e23288. PubMed
PMID: 23525955; PubMed Central PMCID: PMC3601183.
21: Munir S, Andersen GH, Met Ö, Donia M, Frøsig TM, Larsen SK, Klausen TW, Svane
IM, Andersen MH. HLA-restricted CTL that are specific for the immune checkpoint
ligand PD-L1 occur with high frequency in cancer patients. Cancer Res. 2013 Mar
15;73(6):1764-76. doi: 10.1158/0008-5472.CAN-12-3507. Epub 2013 Jan 17. PubMed
PMID: 23328583.
22: Kloke BP, Kutscher S, Rae R, Kvistborg P, Britten CM, Hadrup SR. Toward
next-generation cancer immunotherapy: 10th annual meeting of the Association for
Cancer Immunotherapy (CIMT), Mainz, Germany, May 23-25, 2012. Cancer Immunol
Immunother. 2013 May;62(5):975-81. doi: 10.1007/s00262-012-1386-2. Epub 2013 Jan
- PubMed PMID: 23299564.
23: Chang CX, Tan AT, Or MY, Toh KY, Lim PY, Chia AS, Froesig TM, Nadua KD, Oh
HL, Leong HN, Hadrup SR, Gehring AJ, Tan YJ, Bertoletti A, Grotenbreg GM.
Conditional ligands for Asian HLA variants facilitate the definition of CD8+
T-cell responses in acute and chronic viral diseases. Eur J Immunol. 2013
Apr;43(4):1109-20. doi: 10.1002/eji.201243088. Epub 2013 Feb 14. PubMed PMID:
23280567; PubMed Central PMCID: PMC3655610.
24: Munir S, Frøsig TM, Hansen M, Svane IM, Andersen MH. Characterization of
T-cell responses against IκBα in cancer patients. Oncoimmunology. 2012 Nov
1;1(8):1290-1296. PubMed PMID: 23243592; PubMed Central PMCID: PMC3518501.
25: Hadrup SR. The antigen specific composition of melanoma tumor infiltrating
lymphocytes? Oncoimmunology. 2012 Sep 1;1(6):935-936. PubMed PMID: 23162762;
PubMed Central PMCID: PMC3489750.
26: Ellebaek E, Iversen TZ, Junker N, Donia M, Engell-Noerregaard L, Met Ö,
Hölmich LR, Andersen RS, Hadrup SR, Andersen MH, thor Straten P, Svane IM.
Adoptive cell therapy with autologous tumor infiltrating lymphocytes and low-dose
Interleukin-2 in metastatic melanoma patients. J Transl Med. 2012 Aug 21;10:169.
doi: 10.1186/1479-5876-10-169. PubMed PMID: 22909342; PubMed Central PMCID:
PMC3514199.
27: Kvistborg P, Shu CJ, Heemskerk B, Fankhauser M, Thrue CA, Toebes M, van Rooij
N, Linnemann C, van Buuren MM, Urbanus JH, Beltman JB, Thor Straten P, Li YF,
Robbins PF, Besser MJ, Schachter J, Kenter GG, Dudley ME, Rosenberg SA, Haanen
JB, Hadrup SR, Schumacher TN. TIL therapy broadens the tumor-reactive CD8(+) T
cell compartment in melanoma patients. Oncoimmunology. 2012 Jul 1;1(4):409-418.
PubMed PMID: 22754759; PubMed Central PMCID: PMC3382882.
28: Andersen RS, Kvistborg P, Frøsig TM, Pedersen NW, Lyngaa R, Bakker AH, Shu
CJ, Straten Pt, Schumacher TN, Hadrup SR. Parallel detection of antigen-specific
T cell responses by combinatorial encoding of MHC multimers. Nat Protoc. 2012 Apr
12;7(5):891-902. doi: 10.1038/nprot.2012.037. PubMed PMID: 22498709.
29: Ellebaek E, Engell-Noerregaard L, Iversen TZ, Froesig TM, Munir S, Hadrup SR,
Andersen MH, Svane IM. Metastatic melanoma patients treated with dendritic cell
vaccination, Interleukin-2 and metronomic cyclophosphamide: results from a phase
II trial. Cancer Immunol Immunother. 2012 Oct;61(10):1791-804. Epub 2012 Mar 20.
PubMed PMID: 22426890.
30: Andersen RS, Thrue CA, Junker N, Lyngaa R, Donia M, Ellebæk E, Svane IM,
Schumacher TN, Thor Straten P, Hadrup SR. Dissection of T-cell antigen
specificity in human melanoma. Cancer Res. 2012 Apr 1;72(7):1642-50. doi:
10.1158/0008-5472.CAN-11-2614. Epub 2012 Feb 6. PubMed PMID: 22311675.
31: Brimnes MK, Gang AO, Donia M, Thor Straten P, Svane IM, Hadrup SR. Generation
of autologous tumor-specific T cells for adoptive transfer based on vaccination,
in vitro restimulation and CD3/CD28 dynabead-induced T cell expansion. Cancer
Immunol Immunother. 2012 Aug;61(8):1221-31. doi: 10.1007/s00262-011-1199-8. Epub
2012 Jan 12. PubMed PMID: 22237888.
32: Hombrink P, Hadrup SR, Bakker A, Kester MG, Falkenburg JH, von dem Borne PA,
Schumacher TN, Heemskerk MH. High-throughput identification of potential minor
histocompatibility antigens by MHC tetramer-based screening: feasibility and
limitations. PLoS One. 2011;6(8):e22523. doi: 10.1371/journal.pone.0022523. Epub
2011 Aug 5. PubMed PMID: 21850230; PubMed Central PMCID: PMC3151248.
33: Sørensen RB, Hadrup SR, Svane IM, Hjortsø MC, Thor Straten P, Andersen MH.
Indoleamine 2,3-dioxygenase specific, cytotoxic T cells as immune regulators.
Blood. 2011 Feb 17;117(7):2200-10. doi: 10.1182/blood-2010-06-288498. Epub 2010
Nov 15. PubMed PMID: 21079151; PubMed Central PMCID: PMC3062329.
34: Trepiakas R, Berntsen A, Hadrup SR, Bjørn J, Geertsen PF, Straten PT,
Andersen MH, Pedersen AE, Soleimani A, Lorentzen T, Johansen JS, Svane IM.
Vaccination with autologous dendritic cells pulsed with multiple tumor antigens
for treatment of patients with malignant melanoma: results from a phase I/II
trial. Cytotherapy. 2010 Oct;12(6):721-34. doi: 10.3109/14653241003774045. PubMed
PMID: 20429791.
35: Hadrup SR, Schumacher TN. MHC-based detection of antigen-specific CD8+ T cell
responses. Cancer Immunol Immunother. 2010 Sep;59(9):1425-33. doi:
10.1007/s00262-010-0824-2. Epub 2010 Feb 23. Review. PubMed PMID: 20177676;
PubMed Central PMCID: PMC2892606.
36: Sørensen RB, Berge-Hansen L, Junker N, Hansen CA, Hadrup SR, Schumacher TN,
Svane IM, Becker JC, thor Straten P, Andersen MH. The immune system strikes back:
cellular immune responses against indoleamine 2,3-dioxygenase. PLoS One. 2009 Sep
7;4(9):e6910. doi: 10.1371/journal.pone.0006910. PubMed PMID: 19738905; PubMed
Central PMCID: PMC2734165.
37: Kwong GA, Radu CG, Hwang K, Shu CJ, Ma C, Koya RC, Comin-Anduix B, Hadrup SR,
Bailey RC, Witte ON, Schumacher TN, Ribas A, Heath JR. Modular nucleic acid
assembled p/MHC microarrays for multiplexed sorting of antigen-specific T cells.
J Am Chem Soc. 2009 Jul 22;131(28):9695-703. doi: 10.1021/ja9006707. PubMed PMID:
19552409; PubMed Central PMCID: PMC2720314.
38: Hadrup SR, Bakker AH, Shu CJ, Andersen RS, van Veluw J, Hombrink P,
Castermans E, Thor Straten P, Blank C, Haanen JB, Heemskerk MH, Schumacher TN.
Parallel detection of antigen-specific T-cell responses by multidimensional
encoding of MHC multimers. Nat Methods. 2009 Jul;6(7):520-6. doi:
10.1038/nmeth.1345. Epub 2009 Jun 21. PubMed PMID: 19543285.
39: Køllgaard T, Duval L, Schmidt H, Kaltoft K, Seremet T, Andersen MH, Maase Hv,
Straten Pt, Hadrup SR. Longitudinal immune monitoring of patients receiving
intratumoral injection of a MART-1 T-cell receptor-transduced cell line (C-Cure
709). Cytotherapy. 2009;11(5):631-41. doi: 10.1080/14653240902923146. PubMed
PMID: 19530030.
40: Hadrup SR, Toebes M, Rodenko B, Bakker AH, Egan DA, Ovaa H, Schumacher TN.
High-throughput T-cell epitope discovery through MHC peptide exchange. Methods
Mol Biol. 2009;524:383-405. doi: 10.1007/978-1-59745-450-6_28. PubMed PMID:
19377960.
41: Sørensen RB, Andersen RS, Svane IM, Engell-Noerregaard L, Hadrup SR, Balslev
E, Andersen MH, thor Straten P. CD8 T-cell responses against cyclin B1 in breast
cancer patients with tumors overexpressing p53. Clin Cancer Res. 2009 Mar
1;15(5):1543-9. doi: 10.1158/1078-0432.CCR-08-1412. Epub 2009 Feb 17. PubMed
PMID: 19223507.
42: Kvistborg P, Hadrup SR, Svane IM, Andersen MH, Straten PT. Characterization
of a single peptide derived from cytochrome P450 1B1 that elicits spontaneous
human leukocyte antigen (HLA)-A1 as well as HLA-B35 restricted CD8 T-cell
responses in cancer patients. Hum Immunol. 2008 Apr-May;69(4-5):266-72. doi:
10.1016/j.humimm.2008.02.007. Epub 2008 Apr 4. PubMed PMID: 18486761.
43: Kollgaard T, Hadrup SR, Petersen SL, Masmas TN, Andersen MH, Spierings E,
Vindeløv L, Straten PT. Natural T-cell responses against minor histocompatibility
antigen (mHag) HY following HLA-matched hematopoietic cell transplantation: what
are the requirements for a ‘good’ mHag? Leukemia. 2008 Oct;22(10):1948-51. doi:
10.1038/leu.2008.75. Epub 2008 Apr 10. PubMed PMID: 18401420.
44: Bakker AH, Hoppes R, Linnemann C, Toebes M, Rodenko B, Berkers CR, Hadrup SR,
van Esch WJ, Heemskerk MH, Ovaa H, Schumacher TN. Conditional MHC class I ligands
and peptide exchange technology for the human MHC gene products HLA-A1, -A3,
-A11, and -B7. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3825-30. doi:
10.1073/pnas.0709717105. Epub 2008 Feb 28. PubMed PMID: 18308940; PubMed Central
PMCID: PMC2268811.
45: Rodenko B, Toebes M, Hadrup SR, van Esch WJ, Molenaar AM, Schumacher TN, Ovaa
- Generation of peptide-MHC class I complexes through UV-mediated ligand
exchange. Nat Protoc. 2006;1(3):1120-32. PubMed PMID: 17406393.
46: Sørensen RB, Hadrup SR, Køllgaard T, Svane IM, thor Straten P, Andersen MH.
Efficient tumor cell lysis mediated by a Bcl-X(L) specific T cell clone isolated
from a breast cancer patient. Cancer Immunol Immunother. 2007 Apr;56(4):527-33.
Epub 2006 Jul 19. PubMed PMID: 16850344.
47: Hadrup SR, Gehl J, Sørensen RB, Geertsen PF, Straten PT, Andersen MH.
Persistence of survivin specific T cells for seven years in a melanoma patient
during complete remission. Cancer Biol Ther. 2006 May;5(5):480-2. Epub 2006 May
- PubMed PMID: 16582593.
48: Hadrup SR, Braendstrup O, Jacobsen GK, Mortensen S, Pedersen LØ, Seremet T,
Andersen MH, Becker JC, Straten PT. Tumor infiltrating lymphocytes in seminoma
lesions comprise clonally expanded cytotoxic T cells. Int J Cancer. 2006 Aug
15;119(4):831-8. PubMed PMID: 16557580.
49: Hadrup SR, Strindhall J, Køllgaard T, Seremet T, Johansson B, Pawelec G, thor
Straten P, Wikby A. Longitudinal studies of clonally expanded CD8 T cells reveal
a repertoire shrinkage predicting mortality and an increased number of
dysfunctional cytomegalovirus-specific T cells in the very elderly. J Immunol.
2006 Feb 15;176(4):2645-53. PubMed PMID: 16456027.
50: Kollgaard T, Petersen SL, Hadrup SR, Masmas TN, Seremet T, Andersen MH,
Madsen HO, Vindeløv L, thor Straten P. Evidence for involvement of clonally
expanded CD8+ T cells in anticancer immune responses in CLL patients following
nonmyeloablative conditioning and hematopoietic cell transplantation. Leukemia.
2005 Dec;19(12):2273-80. PubMed PMID: 16304575.
