Immune Monitoring

• Monitoring by flow cytometry of specific T cell responses restricted to MHC class I or II molecules through the use of MHC multimers. We offer immune monitoring with the use of GMP certified MHC Dextramers. Further, we offer ELISpot as a readout for detection of specific T cell responses.
• Phenotypical analysis of T cell memory subsets, including effector memory, central memory and naïve T cells.
• ELISpot- or flow cytometry-based analysis of intracellular cytokine production, including, but not limited to effector and regulatory cytokines such as IFN-γ, TNF-α, IL-2, IL-10, IL-17 or TGF-β in response to stimulation with e.g. peptides, antigens or cell lines.
• Analysis of regulatory CD4 or CD8 T cells.
• Analysis of frequency and functionality of myeloid-derived suppressor cells (MDSC).
• Analysis of the frequency of dendritic cell subsets.
• Analysis of the frequency of other immune cell subsets such as Natural Killer cells, Natural killer T cells (NKT cells) and B cells.

Several measurements can be combined in a single experiments, enabling measurement of multiple parameters.

In collaboration with you we will design the experiments in such a way that you get clear, usable data and answers to the immunological questions relevant to you.