ImmuMap Services offer flow cytometry based, quality assured immune assesment of customer samples such as blood and PBMC's. We are experts in flow cytometry based monitoring of cellular immune responses to cancer vaccine- and immunotherapy.
Examples of immune monitoring services offered:
Several measurements can be combined in a single experiments, enabling measurement of multiple parameters.
In collaboration with you we will design the experiments in such a way that you get clear, usable data and answers to the immunological questions relevant to you.
Previous reports have indicated upregulation of the cancer-testis antigens (CTAs) by treatment with de-methylating drugs and in parallel other studies have suggested increased immune competence following administration of such drugs. Thus, if patients’ immune reactivity would be partly responsible for the clinical benefit it would possibly be beneficial to combine demethylating treatment with more directed immune boosting such as a vaccine or indirect immune treatments such as an immune checkpoint inhibitor blocker.
In the academic setting we sat out to investigate this effect further by obtaining blood samples prior to treatment and twice every fourth week from a cohort of 17 patients diagnosed with myeloid malignancies and treated with the de-methylating agent 5-Azacytidine (Gang & Frøsig et al., Blood Cancer Journal, 2014).
We investigated three hypotheses on the effect of 5-Azacytidine treatment:
Methods available: combinatorial encoding of MHC multimers, Hadrup & Bakker et al., Nature Methods, 2009; Andersen et al., Nature Protocols, 2012 or DNA-barcoding of MHC multimers, Bentzen et al., Nature Biotechnology, 2016.
Methods available: all kinds of magnetic cell separation available from Miltenyi Biotec with following: VITAL-FR cytotoxicity assay, Stanke et al., Journal of Immunological Methods, 2010; extracellular staining of activation marker CD107a/b Betts et al., Journal of Immunological Methods 2003 and CD137 (Wolfl et al., Blood 2007).
Methods available: established SOPs for measuring several immune effector and suppressor cell populations.
Note, several of the methods mentioned above are patented and licenses are required in order to use these.
ImmuMap Services offer to perform immune monitoring in our laboratory or to train or advice you on how to perform these studies yourself.